Real-Time Analysis of Low-Volatile Metabolites in Breath
What is it?
SUPER SESI is an ionization source specifically optimized for breath analysis on high performance mass spectrometers
The person exhales through a disposable mouth piece, and the regulator passes a constant fraction of the flow to the ionization region
In the ionization region, a nano-Electrospray creates the primary ions, which transfer their charge to the metabolites of interest
Ionized metabolites are efficiently transferred to the mass spectrometer for analysis
SUPER SESI is compatible with main instruments in the market, such as Thermo and Sciex
No fragmentation is produced. This is because SESI does not involve a high energy region. Ultimately, this simplifies the spectra, thus improving the limits of detection
Plug and breath: SUPER SESI incorporates all the components and controls required to start a breath biomarker discovery campaign. It is ready to connect with the MS and start analyzing
Real time analysis: Breath is ionized and analyzed as it is exhaled. No sample preparation is required. This reduces the chances that a foreign confounding effect distorts the data
High sensitivity: From 5·10-4 Bar to 2.7·10-7 Bar, SUPER SESI has been carefully designed toefficiently ionize low volatility metabolites. More different species can be detected
Optimized for high biological significant metabolites: Large molecules have very low volatility. They are produced at very low concentrations, and tend to stick in the walls of the source. SUPER SESI operates at high temperature, integrates high technology silica coatings to reduce chemical interactions, and it is designed to minimize contact with the inner walls
How it Works
Vapors are preheated and mixed with a cloud of protonated water clusters produced by the nano-electrospray.
Vapors are ionized at high pressure to maximize ionization efficiency and ions are transferred to the MS.
SUPER SESI can be applied in the following researches
Disease markers inbreath samples discovered by SUPER SESI
The choice of research institutes
SUPER SESI hasbeen used by numerous research institutes around the world, for example:
University Children’s Hospital Zurich, Switzerland
Universitäts- und Kinderspital beider Basel,Switzerland