Keeping breath samples as intact as possible is important to reduce the chances of accidentally introducing contaminants and confounding variables that could be potentially misinterpreted as biomarkers. On-line breath analysis provides the most direct method of collecting the data, but not all patients can walk to the lab and consciously exhale into SUPER SESI. For these situations, we introduce our off-line analysis solution:

• Breath is collected in a low-out-gassing inert material bag with minimal chemical interaction.
• The bag is rapidly stored in a portable DESAMPLER, where it is kept at a constant temperature, with no moisture losses.
• The DESAMPLER is coupled with SUPER SESI: it introduces the sample as if the subjects were directly exhaling in the SUPER SESI.